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* Strains for DNA propagation
and cloning
* Strains for protein expression |
List |
Maintenance
Preparing
competent bugs
Bacterial
glycerol stocks
Transformation
Background & tips (Hebrew)
Transformation
(PEG)
Quick
transformation.
Transformation
(electrocompetents)
Induction of Expression
IPTG
induced expression
Salt induced expression
Auto-induced expression
Expression for structure determination
Time Estimation for Bacteria Growth
Media
preparation
Protein Purification & Characterization
Choosing
a lysis buffer (from The
Protein Expression and Purification Facility of EMBL)
Methods for
cell lysis
Affinity binding
Running SDS gels
(+ Coomassie staining)
Western blots
(+ ECL)
Conversion of protein weight to molarity and vice versus. |
