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Methods for 

 
 Cell Lysis

Background
Preparation of cell lysates from E. coli (from The Protein Expression and Purification Facility of EMBL)
Choice of lysis buffer and additives (from The Protein Expression and Purification Facility of EMBL)

Protocols

  1. Cell lysis using B-Per
  2. Cell lysis using sonication
  3. Sonication protocol
  4. Inclusion bodies
  5. Complete Lysis-B (protein extraction from bacteria and insect cells - Roche)

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