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Recombinant
DNA lab (an interactive tutorial)
PCR
Strategy (from The
Protein Expression and Purification Facility of EMBL)
Let
your fingers do the walking (an interactive tutorial)
Gel
electrophoresis of DNA and RNA, Multiple
DNA forms in gel,
DNA
modifying enzymes
Setting Up a Restriction Endonuclease Reaction (NEB)
Restriction Endonucleases FAQ (NEB)
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Preparing DNA
Miniprep tips (Hebrew)
PCR Cloning
1. Bio-X-act,
2. Platinum
pfx,
3. Phusion
PCR Screening
1. Colony PCR screening
2.GoTaq Green Master Mix
Ligation
DNA ligation tips (Hebrew)
Direct ligation with
oligo
Modifying enzymes:
1. Klenow,
2. Mung
Bean
Mutagenesis:
1. Phusion
2. Quickchange
3. Quickchange multi
Additional Protocol Sites
1.Molecular biology protocols from Protocol Online)
2. DNA &
protein protocols
3. Molecular station protocols
4. Cold Spring Harbor Protocols
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DNA Manipulation
1. Generating restrictions maps
2. Mapper
3. REbase
4. Silent mutation restriction enzyme scan
5. Translate (nucleotides to protein) A. Expasy B. EMBOSS
6. Reverse translation (protein to nucleotides)
7. Sequence Format Conversion
PCR Design
1. Primer
Tools for PCR
2. Primer Design
3. Nucleic Acids Calculators (Agilent)
4. Reverse
complement
5. Oligo Calc:
6.Spectrophotometric
Measurement of Nucleic Acids' Concentration
7. Weight to Molar
Quantity (for nucleic acids)
Genetic Tools
1. REPK - finds sets of 4 commercially available restriction endonucleases which together uniquely differentiate designated sequence groups from a supplied FASTA format sequence file for use in T-RFLP.
2. PrimerZ -
primer design for genes and human SNPs (PubMed)
Tool Sets for DNA Manipulation
1. everyVECTOR
2. pDRAW32 DNA analysis software
3. DNA Strider (for Mac)
4. EnzymeX
5. APE - A plasmid editor
6. Sequence Manipulation Suite
7. CLC free workbench
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BioSupplyNet
(searching for scientific supplies.)
NEB
(restriction endo-nucleases, table
of double digest, Cleavage
close to the end of DNA fragments Table 1 & Table 2 , Star
activity)
Fermentas
(FastDigest restriction
enzymes: Conventional restriction enzymes.)
Promega (restriction
enzymes resource, Cloning
enzyme, PCR review, PCR products)
Roche Applied Science
(product selection )
Takara
(molecular biology, PCR )
Sigma PCR product guide
Oligos Synthesis (Sigma/Genosis,
Eisenberg Bros, MBC, Rhenium) |