Removal of single stranded extensions using Mung Bean Nuclease

Mung Bean endonuclease  degrades single-stranded extensions from the ends of  DNA and RNA molecules, leaving blunt, ligatable ends.

1. DNA (0.1 ug/ul) should be suspended in either 1xMung bean buffer:

or in  NEB buffers 1, 2 or 4.  If using NEBuffer 1 2 or 4, add ZnSO4 to final conc. Of 1mM
Reaction volume should be 50-100µl
2. Add 1u of enzyme
3. Incubate at 30deg. 30min.
4. Inactivate the enzyme by phenol/chloroform extraction
5. Recover DNA by ethanol precipitation