Protein Production from Academy to Therapy- PPAT (#92632) - 2019
More than a simple His purification and PAGE-SDS analysis is necessary to prepare a protein sample for biophysical, biochemical or clinical studies.
Covered topics:
Basic principles on protein production. Main flowchart
Protein expression. Advantages and disadvantages of different expression systems
Cell disruption considerations. Centrifugation. Filtration. Other basic tools in protein production: Ultrafiltration and other protein concentration methods. Dialysis. Protein quantification: different methods. Protein analysis (PAGE-SDS, native gels, IEF, etc)
Batch purification, open columns, FPLC system. Resolution, efficiency, selectivity and capacity
Ion exchange, Hydrophobic Exchange, Mixed Mode and Gel Filtration Chromatography: Main stages in chromatography. Basis for selectivity. Operational considerations. Determination of start conditions. Parameters for optimization. Other types of chromatographic procedures. Academic and Industrial use of these procedures.
Affinity chromatography: Main stages. Advantages and Disadvantages. Type of Affinities.
Principles on protein characterization. Minimal criteria in Academic and Pharma
Future Trend in Pharma
Difficult targets: membrane proteins, protein complexes, virus, etc
Course will be open for students and under-graduate students (Master, PhD and post-doc) that need highly purified proteins for their studies. Rules for the course First semester (October 2019 – January 2020) every Tuesday between 9:00 to 10:45 - Room 504 - Life Science Institute
29/10 Introduction to Protein Purification. Input for Purification Protocol Development - Main flowchart - Guidelines for Protein Purification - Protein quantification: different methods. Protein analysis (PAGE-SDS, native gels, IEF, etc) Speaker: Dr. Mario Lebendiker(Literature)
12/11Sample Preparation before Chromatography - Cell disruption considerations. Centrifugation. Filtration - Chromatography: General Considerations. Other basic tools in protein production: Ultrafiltration and other protein concentration methods. Dialysis. Speaker: Dr. Mario Lebendiker(Literature)
19/11Ion Exchange: Main stages in chromatography. Basis for selectivity. Operational considerations. Determination of start conditions. Parameters for optimization. Example Speaker: Dr. Mario Lebendiker(Literature)
26/11 Gel Filtration Chromatography and desalting: Main stages in chromatography. Basis for selectivity. Operational considerations. Determination of start conditions. Parameters for optimization. Examples. SEC-MALS Speaker: Dr. Mario Lebendiker (Literature)
03/12 Affinity chromatography: Main stages. Advantages and Disadvantages. Type of Affinities. Designing and preparing an affinity gel. Antibody purification for Therapy. Future Trend in Pharma Speaker: Dr. Mario Lebendiker(Literature)
10/12 Genetically engineered Tags, recombinant proteins. Cleavage Sites. Parameters for optimization. Examples. Speaker: Dr. Mario Lebendiker
31/12 How to solve aggregation problems. Mechanism of aggregation. Methods for screening solubility. Considerations when selecting buffer components. Additives that increase yield and prevent aggregation and/or stabilize proteins Speaker: Dr. Mario Lebendiker
07/01 Refolding: Major steps. Refolding methods. Screening. Strategies and examplesSpeaker: Dr. Mario Lebendiker
14/01 Selection and combination of purification techniques. Examples. Storage Speaker: Dr. Mario Lebendiker
21/01 Principles on protein characterization. Major requirements for purification of proteins for structural studies: crystallography, NMR, others. Minimal QC criteria in Academic and Pharma Speaker: Dr. Mario Lebendiker
28/01 The challenge of Membrane protein purification. Main features, use and removal of detergents. Purification of big protein complex, virus, nano-vesicles Speaker: Dr. Mario Lebendiker
Protein Production from Academy to Therapy- PPAT (#92632)
- 2018
More than a simple His purification and PAGE-SDS analysis is necessary to prepare a protein sample for biophysical, biochemical or
clinical studies.
Covered topics:
Basic principles on protein expression. Advantages and disadvantages of different expression systems
Ion exchange, Hydrophobic Exchange, and Gel Filtration Chromatography: Main stages in chromatography. Basis for selectivity. Operational considerations. Determination of start conditions. Parameters for optimization. Other types of chromatographic procedures.
Affinity chromatography: Main stages. Advantages and Disadvantages. Type of Affinities.
Genetically
engineered Tags. Cleavage Sites
Other basic tools in protein production: Ultrafiltration and other protein concentration methods. Dialysis. Protein quantification: different methods. Protein analysis (PAGE-SDS, native gels, IEF, etc)
Principles on protein characterization
Course will be open for students and under-graduate students (Master, PhD and post-doc) that need highly purified proteins for their studies.Rules for the course
Second semester (March - Juny 2018) every Tuesday between 9:00 to 10:45 . Room 504 - Life Science Institute 20/3Introduction to Protein Purification. Input for Purification Protocol Development - Guidelines for Protein Purification Speaker: Dr. Mario Lebendiker (Literature) 27/3Sample Preparation before Chromatography - Cell disruption considerations. Centrifugation. Filtration - Chromatography: General Considerations Speaker: Dr. Mario Lebendiker(Literature)
10/4 Ion Exchange: Main stages in chromatography. Basis for selectivity. Operational considerations. Determination of start conditions. Parameters for optimization. Example Speaker: Dr. Mario Lebendiker(Literature) 17/4 Gel Filtration Chromatography and desalting: Main stages in chromatography. Basis for selectivity. Operational considerations. Determination of start conditions. Parameters for optimization. Examples. SEC-MALS Speaker: Dr. Mario Lebendiker(Literature)24/4 Hydrophobic Exchange Chromatography: Basis for selectivity. Determination of start conditions. Operational considerations. Parameters for optimization. Examples. (Literature) Other Purification Techniques: Multimode Resins (HIC + IEX & GF + IEX), Hydroxyapatite, Salt tolerant, Reverse Phase and more (Literature) Speaker: Dr. Mario Lebendiker 01/5 Affinity chromatography: Main stages. Advantages and Disadvantages. Type of Affinities. Designing and preparing an affinity gel Speaker: Dr. Mario Lebendiker(Literature) 08/5 Genetically engineered Tags, recombinant proteins. Cleavage Sites. Parameters for optimization. Examples Speaker: Dr. Mario Lebendiker
15/5 Selection and combination of purification techniques. Examples. StorageSpeaker: Dr. Mario Lebendiker
22/5 Basic principles on protein expression and bacterial systemsSpeaker: Dr. Tsafi Danieli 29/5 Mammalian Cell expression Speaker: Dr. Tsafi Danieli 05/6 How to solve aggregation problems. Mechanism of aggregation. Methods for screening solubility. Considerations when selecting buffer components. Additives that increase yield and prevent aggregation and/or stabilize proteinsSpeaker: Dr. Mario Lebendiker
12/6 Refolding: Major steps. Refolding methods. Screening. Strategies and examples Speaker: Dr. Mario Lebendiker 19/6 Principles on protein characterization. Major requirements for purification of proteins for structural studies: crystallography, NMR, others Speaker: Dr. Mario Lebendiker
26/6 The challenge of Membrane protein purification. Main features, use and removal of detergentsSpeaker: Dr. Mario Lebendiker
Protein Production for
Biophysical and Biochemical Studies - PPBBS (#92632)
- 2015 More than a simple His purification and PAGE-SDS analysis is necessary to prepare a protein sample for biophysical, biochemical or
clinical studies.
Covered topics:
Basic principles on protein expression. Advantages and disadvantages of different expression systems
Ion exchange, Hydrophobic Exchange, and Gel Filtration Chromatography: Main stages in chromatography. Basis for selectivity. Operational considerations. Determination of start conditions. Parameters for optimization. Other types of chromatographic procedures.
Affinity chromatography: Main stages. Advantages and Disadvantages. Type of Affinities.
Genetically
engineered Tags. Cleavage Sites
Other basic tools in protein production: Ultrafiltration and other protein concentration methods. Dialysis. Protein quantification: different methods. Protein analysis (PAGE-SDS, native gels, IEF, etc)
Principles on protein characterization
Course will be open for students and under-graduate students (Master, PhD and post-doc) that need highly purified proteins for their studies.Rules for the course
First semester (October 2015 - January 2016) every Wednesday between 9:00 to 10:45 . Room 504 - Life Science Institute.
Basic principles on protein expression. Advantages and disadvantages of different expression systems (second talk)
Course92632_2014/Talks 2015/BacterialExpression2015.pdf Speaker: Dr. Tsafi Danieli (HUJI)18/11/2015Ion Exchange: Main stages in chromatography. Basis for selectivity. Operational considerations. Determination of start conditions. Parameters for optimization. Examples.Speaker: Dr. Mario Lebendiker (HUJI)(Literature)25/11/2015Hydrophobic Exchange: Chromatography: Main stages in chromatography. Basis for selectivity. Determination of start conditions.
Operational considerations. Parameters for optimization. Examples Speaker: Dr. Mario Lebendiker (HUJI)(Literature)Other Purification Techniques: Hydroxyapatite, Reverse Phase, Multimode Resins (HIC + IEX & GF + IEX) Speaker: Dr. Mario Lebendiker (HUJI)(Literature)
2/12/2015Gel Filtration Chromatography and desalting: Main stages in chromatography. Basis for selectivity. Operational considerations. Determination of start conditions. Parameters for optimization. Examples. SEC-MALSSpeaker: Hadar Amartely (HUJI) (Literature)9 and 16/12/2015Affinity chromatography: Type of Affinities. Main stages. Advantages and Disadvantages.Genetically engineered Tags, recombinant proteins. Cleavage Sites. Parameters for optimization. Examples. (Literature)
Speaker: Dr. Mario Lebendiker (HUJI)
23/12/2015
Purification Strategy: Selection and combination of purification techniques. Examples. Protein Characterization. Storage.
Speaker: Dr. Mario Lebendiker (HUJI)
30/12/2015
Aggregation: How to solve aggregation problems. Mechanism of aggregation. Methods for screening solubility. Considerations when selecting buffer components.
Additives that increase yield and prevent aggregation and/or stabilize proteins.
Speaker: Dr. Mario Lebendiker (HUJI) (Literature)
6/01/2015Quality control: Major requirements for purification of proteins for structural studies (crystallography, NMR, others)
Speaker: Dr. Mario Lebendiker (HUJI)(Literature)13/01/2015Refolding: Major steps. Protein refolding methods. Screening. Strategies and examples. Speaker: Dr. Mario Lebendiker (HUJI)(Literature)Membrane Proteins: The challenge of Membrane protein purification. Main features, use and removal of detergents.
Practical aspects of over-expressing bacterial membrane proteins for structural studies. Detergents in crystallography Speaker: Dr. Mario Lebendiker (HUJI)(Literature)
Protein Production for
Biophysical and Biochemical Studies -PPBBS (#92632)
- 2014
Practical
Course (#92638) More than a simple His purification and PAGE-SDS analysis is necessary to prepare a protein sample for biophysical, biochemical or
clinical studies.
The
Protein Purification Facility of the Hebrew University is organizing a course
that comprises basic knowledge in:
ØFPLC
system. FPLC software ØClassical
chromatography: IEX, SEC, HIC, Affinity and Mixed Mode. Main stages. Basis for
selectivity. Operational considerations. Start conditions. Parameters for
optimizationØGenetically
engineered Tags. Cleavage SitesØAdditional
techniques use in protein productionØProtein
purification strategy and protocol development. Connection between expression
and protein quality. Selection and combination of purification techniques ØMajor
requirements for purification of proteins for structural studies and others. ØProtein
quality and characterization: SPR, ITC, MST, AUC, SEC-MALS, etc ØHow
to solve aggregation problems. Refolding. The challenge of membrane protein
purification, etc
Course will be open for 40 students and
under-graduate students (Master, PhD and post-doc) that need highly purified
proteins for their studies. Students from all Israel universities are
invited
to participate.
Four days course (Two credit points):
6/11 20/11 4/12 & 18/12/2014 from 10:00 to 18:00. 10:00 – 10:50 10’ interval
11:00 – 11:50 70’ interval 13:00 – 13:50 10’ interval 14:00 – 14:50 40’ interval 15:30 – 16:20 10’ interval
16:30 – 17:30 end
Optional Practical Course (#92638): at the end of
the Course #92632 students are invited to perform a practical short course in
our Facility working in their own project
1st
day (06/11/2014)
1)10:00 – 10:50
Introduction to Protein Purification. Main stages. Speaker: Dr. Mario Lebendiker (HUJI)(Literature)
2)11:00 – 11:50FPLC system. FPLC software Speaker: Dr. Mario Lebendiker (HUJI)(Literature)3)13:00 – 13:50
Ion Exchange: Main stages in chromatography. Basis for selectivity.
Operational considerations. Determination of
start
conditions. Parameters for
optimization. Speaker: Dr. Mario Lebendiker (HUJI)(Literature)4)14:00 – 14:50Ion Exchange(continue), examples Speaker: Dr. Mario Lebendiker (HUJI)5)15:30 – 16:20 Hydrophobic Exchange: Chromatography and desalting: Main stages in chromatography.
Basis for selectivity.
Determination of
start
conditions.
Operational considerations. Parameters for optimization. Examples. Speaker: Dr. Mario Lebendiker (HUJI)(Literature)
6)16:30 – 17:30 Protein concentration: native methods,
denaturative methods. Protein determination: different methods. Speaker: Ofrah Blatt (HUJI)
2nd
day (20/11/2014)
7)10:00 – 10:50 Gel Filtration Chromatography and desalting: Main stages in
chromatography. Basis for selectivity.
Operational
considerations. Determination
of start conditions. Parameters for
optimization. Examples.
Speaker: Dr. Mario
Lebendiker (HUJI)(Literature)
8)11:00 – 11:50 Hydrophobic Exchange: Chromatography and desalting: Main stages in chromatography.
Basis for selectivity.
Determination of
start
conditions.
Operational considerations. Parameters for optimization. Examples. Speaker: Dr. Mario Lebendiker (HUJI)(Literature)
Other
Purification Techniques: Hydroxyapatite, Reverse Phase, Multimode
Resins (HIC + IEX & GF + IEX) Speaker: Dr. Mario Lebendiker (HUJI)(Literature)9)13:00 – 13:50 Affinity chromatography: An historical introduction. Speaker: Dr. Meir Wilchek (WIS)
10)14:00 – 14:50 Affinity chromatography: Main stages. Advantages and Disadvantages. Type
of Affinities.
Designing and
preparing an affinity gel. Speaker: Dr. Mario Lebendiker (HUJI)(Literature)11)15:30 – 16:20 Affinity chromatography: Genetically engineered Tags, recombinant
proteins. Cleavage Sites.
Parameters for
optimization. Examples. Speaker: Dr. Mario Lebendiker (HUJI)(Literature)
13)10:00 – 10:50 Cell disruption considerations. Centrifugation. Filtration.Columns, membranes.
Speaker: Dr.
Mario Lebendiker (HUJI)
14)11:00 – 11:50 Input for Purification Protocol Development. Guidelines for Protein
Purification. Speaker: Dr. Mario Lebendiker (HUJI)15)13:00 – 13:50Selection and combination of purification techniques. Storage. Speaker: Dr. Mario Lebendiker (HUJI)16)14:00 – 14:50How to solve aggregation problems. Mechanism of aggregation. Methods for
screening solubility.
Considerations when
selecting buffer
components.Additives
that increase yield and prevent aggregation and/or
stabilize
proteins. Speaker: Dr. Mario Lebendiker (HUJI)(Literature)17)15:30 – 16:20 Refolding: Major steps. Protein
refolding methods. Screening. Strategies and examples. Speaker: Dr. Mario Lebendiker (HUJI)(Literature)18)16:30 – 17:30The challenge of Membrane protein
purification. Main features, use and removal of detergents. Speaker: Dr. Mario Lebendiker (HUJI)(Literature)
4th
day (18/12/2014)
19)10:00 – 10:50Advantages and Disadvantages of different
expression systems. Connection
between expression and
protein quality. Speaker: Prof Gali Prag (TAU)(Literature)
20)11:00 – 11:50 Purification of protein complexes (case-study:
oligomeric chaperone). Speaker: Prof. Abdussalam Azem (TAU)
21)13:00 – 13:50Protein-protein interaction. Advantages and disadvantages of SPR, ITC, MST, AUC.
Speaker: Prof. Gideon Schreiber (WIS)
22)14:00 – 14:50 Protein-protein interaction. Advantages and disadvantages of SPR, ITC, MST, AUC.
Speaker: Prof. Gideon Schreiber (WIS)
23)15:30 – 16:20Challenge in the production of biosimilars. Speaker: Dr. Joel Van Gelder (Insight Biopharmaceuticals)
24)16:30 – 17:30 Major requirements for purification of proteins for structural studies:
crystallography, NMR, others.
Speaker: Dr. Mario Lebendiker (HUJI)(Literature)
Membrane proteins:
Practical aspects of over-expressing bacterial
membrane proteins for structural studies.
Detergents in
crystallography. Speaker: Dr. Mario Lebendiker (HUJI)
GE-HEALTHCARE Strategies
for Protein Purification Handbook
(pdf)
GE-HEALTHCARE Protein
purification
Applications (pdf)
GE-HEALTHCARE Protein
and Peptide Purification - Technique selection guide (pdf)
GE-HEALTHCARE
Purifying Challenging Proteins: Membrane proteins, Multiprotein complexes and
Inclusion bodies (pdf)
GE-HEALTHCARE ÄKTAdesign Purification Method
handbook. ÄKTAexplorer™ or ÄKTApurifier™ chromatography (pdf)
GE-HEALTHCARE Purification
of tagged proteins (pdf)
TOSOH: General Principles of Chromatography (pdf) Mario LebendikerWebsiteTable of ContentRecommended LinksSelected Protocols Latest approaches for efficient protein production in drug discovery. Review - Toshihiko Sugiki et.al. - Expert Opin. Drug Discov. (2014) 9(10)(pdf)
Recombinant protein expression in Escherichia coli: advances and challenges - G.Rosano et. al.- Frontiers in microbiology 2014 | Volume5 | Article 172(pdf)
Challenges and opportunities in the purification of recombinant tagged proteins - A.Pina et. al. - Biotechnol Adv (2013), http://dx.doi.org/10.1016/j.biotechadv.2013.12.001(pdf)
High-throughput production of human proteins for crystallization: The SGC experience - P. Savitsky et al. - J. Struct. Biol. (2010), doi:10.1016/j.jsb.2010.06.008(pdf)
Preparative Purification of Recombinant Proteins: Current Status and Future Trends. Review Article - M. Saraswat - (2013) http://dx.doi.org/10.1155/2013/312709(pdf)
Literature FPLC
GE-HEALTHCARE ÄKTAdesign Purification Method
handbook. ÄKTAexplorer™ or ÄKTApurifier™ chromatography (pdf)
GE-HEALTHCARE Packing of Gel Filtration column (movie)
GE-HEALTHCARE Packing of IEX, Affinity or HIC column
(movie)
GE-HEALTHCARE Column
evaluation (movie)
Literature IEX GE-Healthcare: Ion Exchange Chromatography & Chromatofocusing (pdf) TOSOH: Ion Exchange Chromatography (pdf) Separation of Protein Charge Variants with Induced pH
Gradients Using Anion Exchange Chromatographic Columns T. Pabst - Biotechnol. Prog. 2008, 24, 1096 (pdf)
Systematic generation of buffer systems for pH gradient ion
exchange chromatography and their application F. Kroner
- Journal of Chromatography A, 1285
(2013) (pdf)
Literature GF (SEC)
GE-Healthcare: Gel Filtration - Principles and Methods (pdf) GE-HEALTHCARE Packing of Gel Filtration column (movie) TOSOH: GelFiltration (pdf) Literature Affinity
GE-Healthcare: Affinity Chromatography: Columns and media (pdf) Principles and Methods (pdf) GE-Healthcare: Antibody Purification Handbook (pdf)
Pierce: Protein Immobilization (pdf)
TOSOH: Affinity Chromatography resins (pdf)
EY Lectins (pdf)
SIGMA-Aldrich Lectins Literature TAGs & Recomb Proteins
GE-HEALTHCARE: Recombinant Protein Purification Handbook Principles and Methods (pdf)
GE-HEALTHCARE: GST-Fusion Proteins Handbook (pdf) GE-HEALTHCARE: Purification
of tagged proteins (pdf)
Structural Genomics Consortium Protein
production and purification. Nature Methods 2008, 5: 135-146(pdf-I)(pdf-II)Purification of Recombinant Proteins - Mario Website
Enhancement of soluble protein expression through the use of fusion tags - D.Esposito and D. Chatterjee - Current Opinion in Biotechnology 2006, 17:353–358(pdf)
Making the most of affinity tags - D. Waugh - TRENDS in Biotechnology Vol.23 No.6 June 2005(pdf)
Current strategies for the use of aYnity tags and tag removal for the purifcation of recombinant proteins - J.Arnau et al. - Protein Expression and Purification 48 (2006) 1–13(pdf)
Advances in recombinant protein expression for use in pharmaceutical research - Assenberg R, et al. - Curr Opin Struct Biol (2013), http://dx.doi.org/10.1016/j.sbi.2013.03.008(pdf)
Review. Production of disulfide-bonded proteins in Escherichia coli - M.Berkmen - Protein Expression and Purification 82 (2012) 240(pdf)
Production
of Recombinant Disulfide-Rich Venom Peptides for Structural and
Functional Analysis via Expression in the Periplasm of E. coli
- J. Klint et al. - PLoS ONE 8(5): e63865. doi:10.1371/journal.pone.0063865(pdf)
Statistical approaches to maximize recombinant protein expression in Escherichia coli: A general review - C. Papaneophytou et al. -
Protein Expression and Purification 94 (2014) 22–32(pdf)
Cloning technologies for protein expression and purification - James L Hartley - Current Opinion in Biotechnology 2006, 17:359–366(pdf)
Literature HIC
Theory and Use of HIC in Protein Purification Applications - Justin T. McCue - Methods in Enzymology (2009) 463(pdf)
GE-Healthcare: Hydrophobic Interaction and Reversed Phase Chromatography: Principles and Methods (pdf)
TOSOH: Hydrophobic Interaction Chromatography (pdf)
TOSOH: Hydrophobic
Interaction Chromatography: Effects of Mixed Electrolytes on Protein
Separation (pdf)
Literature Mixed Mode and RPC GE-Healthcare: Hydrophobic Interaction and Reversed Phase Chromatography: Principles and Methods (pdf) GE-Healthcare: Multimodal Chromatography Handbook (pdf) GE-Healthcare: Purification of influenza A/H1N1 using Capto™ Core 700 (pdf) VYDAC The Handbook of Analysis and
Purification of Peptides and Proteins by Reverse-Phase
HPLC (pdf) Multimodal chromatography: Characterization of protein binding and selectivity enhancement through mobile phase modulators L. Wolfe et.al - Journal of Chromatography A, 1340 (2014) 151–156(pdf)
Multimodal chromatography: An efficient tool in downstream processing
of proteins - K. Kallberg et. al - Biotechnol. J. 2012, 7 (pdf)
Evaluation of selectivity in multimodal anion exchange systems: A priori prediction...Y. Hou, S.M. Cramer - J. Chromatogr. A (2011)(pdf)
IgM Purification with Hydroxyapatite - P. Gagnon et. al - BioProcess International 12(2) February 2014(pdf)
Literature Aggregation Review: Production of prone-to-aggregate proteins - M.Lebendiker & T.Danieli - FEBS Letters 588 (2014) 236–246(pdf)
A screening methodology for purifying proteins with aggregation problems. Lebendiker M., Maes M. and Friedler A. (2015)
Methods in Molecular Biology: "Insoluble Proteins" book (Springer)1258: 261-281 (pdf)
Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins - D.Leibly et al. - PLoS ONE 7(12): e52482. doi:10.1371/journal.pone.0052482(pdf)
Molecular origins of surfactant-mediated stabilization of protein drugs - H.Leeet al. - Advanced Drug Delivery Reviews (2011) doi:10.1016/j.addr.2011.06.015(pdf) Effect of Additives on Protein Aggregation - H.Hamada et al. - Current Pharmaceutical Biotechnology, 2009, 10, 400-407 (pdf)
Protein Aggregation: Pathways, Induction Factors and Analysis - H.Mahler et al. - JOURNAL OF PHARMACEUTICAL SCIENCES, VOL. 98 (2009)(pdf)
Mechanisms of Protein Aggregation - J.Philo & and T.Arakawa - Current Pharmaceutical Biotechnology, 2009, 10, 348-351(pdf)
Analytical tools for characterizing biopharmaceuticals and the implications for biosimilars - S. Berkowitz - NATURE REVIEWS | DRUG DISCOVERY VOLUME 11 | JULY 2012 | 527(pdf)
Interactions of formulation excipients with proteins in solution and in the dried state - S.Ohtake et al. - Adv. Drug Deliv. Rev. (2011), doi:10.1016/j.addr.2011.06.011 (pdf)
Classification of Protein Aggregates - L.Narhi et al. - Journal of Pharmaceutical Sciences, Vol. 101, 493–498 (2012) (pdf)
Protein Misfolding and Aggregation Research: Some Thoughts on Improving Quality and Utility - R. Murphy & C. Roberts - Biotechnol. Prog., 2013, Vol. 29, No. 5 (pdf)
Strategies for the Assessment of Protein Aggregates in Pharmaceutical Biotech Product Development - J.Engelsmanet al. - Pharm Res (2011) 28:920–933(pdf)
Protein Aggregation and Bioprocessing - M.Cromwell et al. - The AAPS Journal 2006; 8 (3) Article 66 (http://www.aapsj.org). (pdf)
Molecular level insight into intra-solvent interaction effects on protein stability and aggregation - D. Shukla, et al. - Adv. Drug Deliv. Rev. (2011), doi:10.1016/j.addr.2011.06.014(pdf)
Literature Refolding Protein refolding for industrial processes - E.De Bernardez Clark - Current Opinion in Biotechnology 2001, 12:202–207(pdf)
Preparative protein refolding - A.Middelberg - TRENDS in Biotechnology Vol.20 No.10 October 2002(pdf)
Practical considerations in refolding proteins from inclusion bodies - K.sumoto et al. - Prot. Expr. & Purific. 28 (2003) 1–8(pdf)
In vitro protein refolding by chromatographic procedures - Ming et al. - Protein Expression and Purification 33 (2004) 1–10(pdf)
On-column protein refolding for crystallization - N.Oganesyan et al. - Journal of Struct. & Funct. Genomics 6: 177–182, 2005(pdf) Confronting high-throughput protein refolding using high pressure and solution screens - M.Qoronfleh et al. - Protein Expression and Purification 55 (2007) 209–224(pdf)
Single pH buffer refolding screen for protein from inclusion bodies - B.Coutard et al. - Protein Expr. & Purific. 82 (2012) 352 (pdf)
Protein refolding using chemical refolding additives - S. Yamaguchi et al. - Biotechnol. J. 2013, 8, 17–31(pdf)
Literature Membrane Proteins
Saccharomyces cerevisiae–Based Platform for Rapid Production and Evaluation of Eukaryotic Nutrient Transporters and
Transceptors for Biochemical Studies and Crystallography. - Scharff-Poulsen P, Pedersen PA (2013) - PLoS ONE 8(10): e76851. doi:10.1371/journal.pone.0076851 (pdf)
A general path for large-scale solubilization of cellular proteins: From membrane receptors to multiprotein complexes - F.Pullara et al.- Protein Expression and Purification 87 (2013) 111–119(pdf)(pdf Supl)
High-throughput expression and purification of membrane proteins - F.Mancia et al.- J. Struct. Biol. (2010), doi:10.1016/j.jsb.2010.03.021(pdf)
A Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Protein Precrystallization
Screening - M.Hattori et al.- Structure 20, 1293–1299, August 8, 2012 (pdf)
An efficient strategy for high throughput screening of recombinant integral membrane protein expression and stability - J.Fan et al.- Protein Expr. Purif. (2011), doi:10.1016/j.pep.2011.02.010(pdf)
Detergent selection for enhanced extraction of membrane proteins - B. Arachea et al.- Protein Expression and Purification 86 (2012) 12–20(pdf)
Expression and Purification of Membrane Proteins -J.Kubicek et al.- Methods in Enzymology, Volume 541 (2014) (pdf)
Literature Protein Quality Control
Methods for Protein Characterization by Mass Spectrometry, Thermal Shift (ThermoFluor) Assay, and Multiangle or Static Light
Scattering - J. Nettleship et al. - (2008) Meth.in Molec.Biol., Vol. 426: Struct.Proteom.: High-throughput Methods(pdf)
Impact of Residual Impurities and Contaminants on Protein Stability - W.Wang et al. -
(2014) J.ofPharm.Sc. DOI 10.1002/jps.23931(pdf)
Building better drugs: developing and regulating engineered therapeutic proteins - Kimchi-Sarfatyet. al.- (2013) Trends in Pharmacological Sciences, October 2013, Vol. 34, No. 10 (pdf)
Analytical tools for characterizing biopharmaceuticals and the implications for biosimilars - S. Berkowitz - NATURE REVIEWS | DRUG DISCOVERY VOLUME 11 | JULY 2012 | 527(pdf)
Quality assessment and optimization of purified protein samples: why and how?