in-vitro translation systems

HUJI

Faculty of Science

Cell-Free Translation

General Background Whittaker JW., Cell-free protein synthesis: the state of the art. Biotechnol Lett. 2013 35(2):143-52 Bernhard F, Tozawa Y., Cell-free expression--making a mark. Curr Opin Struct Biol. 2013 23(3):374-80 (With reference to membrane proteins) Sawasaki T. et. al., A cell-free protein synthesis system for high-throughput proteomics , PNAS 2002 99;14652-14657
Product (Manufacturer)	Features & Background	Vectors	Supplements	Additional Information & Protocols
RTS-100 E. coli S30 extract or RTS-500 E. coli S30 extract (5PRIME)	RTS overview (5PRIME) RTS technology pages (5PRIME) RTS tips & tricks (5PRIME)	pIVEX maps & sequences.	1. RTS DnaK supplement , 2. GroE supplement 3. RTS amino acid sampler	Biomax Protein Expression Analysis (Continuation of ProteoExpert)
E coli high yield kits (RINA) E coli disulfide kits (RINA)	Overview of RTS E. coli High Yield System (RINA)	pIVEX vector sets (Data Sheet)	1. RTS DnaK supplement , (data sheet) 2. GroE supplement (data sheet) 3. RTS amino acid sampler
Expressway (E. coli S30) (Invitrogen)	Overview of the Expressway ™ Cell-Free Expression Systems	pExp-Dest GateWay vector kit, pExp3-Dest,		Manual,
Expressway plus (E. coli S30) (Invitrogen)	Accelerate Production of Active Protein with Expressway ™ Plus			Manual,
Insect kits (RINA)	Overview of eukaryotic systems (RINA)
RTS Wheat Germ extract (5PRIME)
Wheat Germ kits (RINA)	Wheat Germ System (RINA)	RTS pIVEX Wheat Germ His6-tag Vector Set
TnT Coupled Reticulocyte Lysate Systems (Promega)	1. In Vitro Expression 2. FAQs (choose Tnt)			Introduction & protocols,

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Entries since May 2004

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