Faculty of Science

Choosing an Expression System

Selected Websites

• Anatomy of an Expression Vector - features affecting expression.
• Movies for laymen (transcription, translation and more..!)
• Co- and post-translational modifications.
• Comparison of expression systems (from The Protein Expression and Purification Facility of EMBL)
• Questions to be asked when choosing an expression system (from The Protein Expression and Purification Facility of EMBL)
• Course 72681 lecture notes (Due to copyrights limitations these presentations can be viewed only by huji users.)

Selected Papers

• Berlec A, Strukelj B., Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells. J Ind Microbiol Biotechnol. 2013 40(3-4):257-274
• Reitz C, Li J, Neubauer P. Recombinant protein production: a comparative view on host physiology (Laupheim, Germany, March 2013) N Biotechnol 2013 30(5):405-409
• Assenberg R, et. al., Advances in recombinant protein expression for use in pharmaceutical research. Curr Opin Struct Biol. 2013 23(3):393-402
• Wang W. et. al., Impact of residual impurities and contaminants on protein stability J Pharm Sci. 2014 103(5):1315-1330
• Bernaudat F. et. al., Heterologous expression of membrane proteins: choosing the appropriate host. PLoS One 2011 6(12):e29191
• Structural Genomics Consortium, Protein Production & Purification (Review) Nature Methods, 2008 5(2):135-146 [PDF]

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Useful Repositories and Collections

Cells

• ATCC - collections of cell lines, bacteria, viruses, fungi and protozoa
• Developmental studies hybridoma bank

Plasmids

• Protein structure initiative vector repository
• Addgene’s collection
• PlasmID - DF/HCC DNA Resource Core
• Additional resources (compiled by Gene Identity)

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Related Products

• Biocompare (the buyers guide for life sciences) products associated with cloning, protein expression & protein purification.
• Biosupplynet Searching for Scientific Supplies.
• Biobuy (Hebrew)

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Related Protocols

• Protocol online (A SCIENCE Recommended Site)
• Cold Spring Harbor protocols
• Protocol database compiled by the Protein Expression and Purification Facility of EMBL, Heidelberg.
• Biotechniques
• Laboratory protocols & methods - free on-line collections
• Vadlo (allows users to search within five categories: Protocols, Online Tools, Seminars, Databases and Software)

This site is maintained by Dr. Nurit Doron . Your comments are most welcome.

entries since October 2003