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Vectors in Use
Vector
Company
Features
Antibioticsr
Documentation
pET28
Novagen
T7 promoter

N or C-terminal His tag.

Kan 
pET28a-c vector map
pET28a sequence
pET28b sequence
pET28c sequence
Parallel vectors
Dr. Peter Sheffield, University of Virginia (UVA)
Parallel vectors 1 & 3 contain N-terminal His (pET22), MBP (pMal) and GST (pGex) with similar MCS for easy cloning.
Parallel vector 2 contains N and C-terminal His (pET22), MBP (pMal) and GST (pGex) with similar MCS for easy cloning.
(The  MCS is compatible to that of pFastBac for expression in insect cells)
Amp
Parallel vectors sequences
pHis1, pHis2, pHis3
pGST1, pGST2, pGST3
pMBP1, pMBP2, pMBP3
pET44a-c
Novagen
T7 promoter, N-term his tag followed by NusA fusion protein, another his-tag, S-tag, thrombin & enterkinase cleavage sites. HSV-tag and his-tags in the C-term are also available, provided a stop codon is not introduced.
Amp
NusA Background

Map
pET44a sequence
pET44b sequence
pET44c sequence

pET43.1a-c
Novagen
T7 promoter, N-term NusA fusion protein followed by his-tag, S-tag, thrombin & enterkinase cleavage sites. HSV-tag and his-tags in the C-term are also available, provided a stop codon is not introduced.
Amp
NusA Background

Map

pIVEX  vectors
(his tag [N,C] GST tag [N], MBP tag [N])
Roche
pIVEX vectors were  developed for in-vitro translation coupled with transcription by T7 polymerase (Roche RTS). Vectors  containing N-term tags have a factor Xa cleavage site. The pIVEX vectors are not IPTG inducible and have a high copy origin. This may result in whole cells in constitutive protein expression which might harm the host cell.
Amp
Maps & Sequences

Vectors manual (PDF)

pDest Gateway 
Invitrogen
For cloning via recombination, for various expression hosts (mammalian, bacterial (T7), insect), and N/C terminal His, Trx and GST fusions, 
Amp
1. Gateway cloning technology - slides set
2. Invitrogen's Gateway main page
3. Gateway cloning technology - tutorial
pGFPuv
Clontech
lac promoter, GFP fluoresence - possible N or C-terminal 
Amp
Vector map +links to sequence
pDsRed2
Clontech
lac promoter, DsRed fluoresence - possible N or C-terminal 
Amp
Vector map +links to sequence
pET22b
 Novagen
 N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His tag.
 Amp
pET22b vector map
pET22b sequence
pEt22FdEK
Alex Fish
N-terminal ferredoxin fusion for increased solubility
Amp
 
pET21d(+)
 Novagen
 
 Amp
pET21a-d vector map
pET21d sequence
pET28pelB
ours
T7 promoter, with a possiblity for C-term his tag, and for periplasmic secretion of the protein
Kan
 

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Entries since October 2007

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