bacterial vectors

Faculty of Science

**Vectors in Use**
Vector	Company	Features	Antibiotics^r	Documentation
pET28	Novagen	T7 promoter N or C-terminal His tag.	Kan	pET28a-c vector map pET28a sequence pET28b sequence pET28c sequence
Parallel vectors	Dr. Peter Sheffield, University of Virginia (UVA)	Parallel vectors 1 & 3 contain N-terminal His (pET22), MBP (pMal) and GST (pGex) with similar MCS for easy cloning. Parallel vector 2 contains N and C-terminal His (pET22), MBP (pMal) and GST (pGex) with similar MCS for easy cloning. (The MCS is compatible to that of pFastBac for expression in insect cells)	Amp	Parallel vectors sequences pHis1, pHis2, pHis3 pGST1, pGST2, pGST3 pMBP1, pMBP2, pMBP3
pET44a-c	Novagen	T7 promoter, N-term his tag followed by NusA fusion protein, another his-tag, S-tag, thrombin & enterkinase cleavage sites. HSV-tag and his-tags in the C-term are also available, provided a stop codon is not introduced.	Amp	NusA Background Map pET44a sequence pET44b sequence pET44c sequence
pET43.1a-c	Novagen	T7 promoter, N-term NusA fusion protein followed by his-tag, S-tag, thrombin & enterkinase cleavage sites. HSV-tag and his-tags in the C-term are also available, provided a stop codon is not introduced.	Amp	NusA Background Map
pIVEX vectors (his tag [N,C] GST tag [N], MBP tag [N])	Roche	pIVEX vectors were developed for in-vitro translation coupled with transcription by T7 polymerase (Roche RTS). Vectors containing N-term tags have a factor Xa cleavage site. The pIVEX vectors are not IPTG inducible and have a high copy origin. This may result in whole cells in constitutive protein expression which might harm the host cell.	Amp	Maps & Sequences Vectors manual (PDF)
pDest Gateway	Invitrogen	For cloning via recombination, for various expression hosts (mammalian, bacterial (T7), insect), and N/C terminal His, Trx and GST fusions,	Amp	1. Gateway cloning technology - slides set 2. Invitrogen's Gateway main page 3. Gateway cloning technology - tutorial
pGFPuv	Clontech	lac promoter, GFP fluoresence - possible N or C-terminal	Amp	Vector map +links to sequence
pDsRed2	Clontech	lac promoter, DsRed fluoresence - possible N or C-terminal	Amp	Vector map +links to sequence
pET22b	Novagen	N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His tag.	Amp	pET22b vector map pET22b sequence
pEt22FdEK	Alex Fish	N-terminal ferredoxin fusion for increased solubility	Amp
pET21d(+)	Novagen		Amp	pET21a-d vector map pET21d sequence
pET28pelB	ours	T7 promoter, with a possiblity for C-term his tag, and for periplasmic secretion of the protein	Kan
Sumo3 Fusion Vector	EMBL	T7 promoter, his tag, TEV site, EGFP	Kan	Map + protocol

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Entries since October 2007