Faculty of Science


Wolfson Home NMR Labrotary X-Ray Labrotary Protein Purification Protein Expression Academic Committee Wolfson  Foundation Wolfson Centre Personnel Wolfson Centre Search

Bacterial  Expression Systems
E coli

Bacterial Strains 
Vectors in Use
Related Procedures & Tools
* Strains for DNA propagation and cloning

* Strains for protein expression



  • Preparing competent bugs
  • Bacterial glycerol stocks


  • Background & tips (Hebrew)
  • Transformation (PEG)
  •  Quick transformation
  • Transformation (electrocompetents)

    Induction of Expression 
  • IPTG induced expression
  • Salt induced expression
  • Auto-induced expression
  • Expression for structure determination
  • Time Estimation for Bacteria Growth
  • Media preparation

    Protein Purification & Characterization
  • Choosing a lysis buffer (from The Protein Expression and Purification Facility of EMBL)
  • Methods for cell lysis
  • Affinity binding
  • Running SDS gels (+ Coomassie staining)
  • Western blots (+ ECL)
  • Conversion of protein weight to molarity and vice versus.

  • This site is maintained by Dr. Nurit Doron . Your comments are most welcome.

    Entries since October 2006

    Copyright ©, 1997, The Hebrew University of Jerusalem. All Rights Reserved