Bacterial strains

**Bacterial Strains for DNA Propagation & Cloning**
Bacterial Strain	Company	Uses	Growth requirements/ Antibiotics^r	Documentation
DH5a	Invitrogen Clontech		N one	Invitrogen's manual
Able C	Stratagene	Reduces copy number of plasmid for toxic proteins	Kan15 mgr/ml	A short description
TG-1	Clontech
Sure2	Stratagene	For increased stability of DNA with secondary structures - this strain prevents DNA rearrangments.	Kan15 mgr/ml Tet12.5 mgr/ml Cam34 mgr/ml	A short description
DM-1	Invitrogen	To achieve unmethylated DNA, when problems with partial restriction enzymes activity are observed	Grow in TB buffer (Specified in Description + Protocols )	Description + Protocols A detailed table listing methylation sensitivity of restriction endonucleases in Nucleic Acids Res. 1991 Apr 25;19 Suppl:2045-71 (PDF)
DB3.1	Invirtogen (BRL)	For production of “Gateway”vectors pDest and pDonor vectors containing the toxic gene ccdB	None	A short description
Topp-10	Invirtogen	Cloning of large fragments of DNA or into large vectors (derived from DH10 cells)
NovaBlue	Novagen	K12 strain for cloning with high transformation efficiency, blue-white screening capability, olso contains DE3 for T7 polymerase expression.	Tet12.5 mgr/ml	A short description
Fusion Blue	BD Biosciences (Clontech)	K-12 strain that provides high transformation efficiency paired with blue/white screening capability when used with the appropriate plasmids. The strain carries recA and endA mutations that make it a good host for obtaining high yields of plasmid DNA. Recommended for use with the In-Fusion and Creator cloning systems.	Optional: Tet12.5 mgr/ml Heat shock during transformation should last only 30 sec.
Stbl2	Invirtogen	A derivative of JM109. The mcrA mutation and the mcrBC-hsdRMS-mrr deletion allow cloning of genomic sequences which are methylated. These cells are not capable of blue/white selection with plasmids containing alpha complementation sequences.	Use of a 30°C expression and incubation temperature is important. Heat shock during transformation should last only 25 sec. Stbl2™ Competent Cells which have been transansformed with plasmids should be grown at 30°C overnight in TB	Detailed protocols
Stbl3 (Site)	Invirtogen	The Stbl3™ E. coli strain is derived from the HB101 E. coli strain and is recommended for use when cloning unstable inserts such as lentiviral DNA containing direct repeats		Protocol

Bacterial Strains for DNA Propagation & Cloning

Bacterial Strain

Company

Uses

Growth requirements/
Antibiotics^r

Documentation

DH5a

Invitrogen
Clontech

N one

Invitrogen's manual

Able C

Stratagene

Reduces copy number of plasmid for toxic proteins

Kan15 mgr/ml

A short description

TG-1

Clontech

Sure2

Stratagene

For increased stability of DNA with secondary structures - this strain prevents DNA rearrangments.

Kan15 mgr/ml
Tet12.5 mgr/ml
Cam34 mgr/ml

A short description

DM-1

Invitrogen

To achieve unmethylated DNA, when problems with partial restriction enzymes activity are observed

Grow in TB buffer (Specified in Description + Protocols )

Description + Protocols

A detailed table listing methylation sensitivity of restriction endonucleases in Nucleic Acids Res. 1991 Apr 25;19 Suppl:2045-71 (PDF)

DB3.1

Invirtogen (BRL)

For production of “Gateway”vectors pDest and pDonor vectors containing the toxic gene ccdB

None

A short description

Topp-10

Invirtogen

Cloning of large fragments of DNA or into large vectors (derived from DH10 cells)

NovaBlue

Novagen

K12 strain for cloning with high transformation efficiency, blue-white screening capability, olso contains DE3 for T7 polymerase expression.

Tet12.5 mgr/ml

A short description

Fusion Blue

BD Biosciences (Clontech)

K-12 strain that provides high transformation efficiency paired with blue/white screening capability when used with the appropriate plasmids. The strain carries recA and endA mutations that make it a good host for obtaining high yields of plasmid DNA. Recommended for use with the In-Fusion and Creator cloning systems.

Optional: Tet12.5 mgr/ml

Heat shock during transformation should last only 30 sec.

Stbl2

Invirtogen

A derivative of JM109. The mcrA mutation and the mcrBC-hsdRMS-mrr deletion allow cloning of genomic sequences which
are methylated. These cells are not capable of blue/white selection with
plasmids containing alpha complementation sequences.

Use of a 30°C expression and incubation temperature is important.

Heat shock during transformation should last only 25 sec.

Stbl2™ Competent Cells which have been transansformed with plasmids should be grown at 30°C overnight in TB

Detailed protocols

Stbl3
(Site)

Invirtogen

The Stbl3™ E. coli strain is derived from the HB101 E. coli strain and is recommended for use when cloning unstable inserts such as lentiviral DNA containing direct repeats

Protocol

**Search for Additional Bacterial Strains**
Novagen	Selection Table of Novagen's Competent Cells Competent cells & Protocols (PDF) Competent Cells Overview
Invitrogen	Competent Cells, Table of competent cells
Stratagene	Competent Cells
Clontech	Electrocompetent & Chemically Competent E. coli and Yeast Cells
Takara	E. coli Competent Cells

Search for Additional Bacterial Strains

Novagen

Selection Table of Novagen's Competent Cells
Competent cells & Protocols (PDF)
Competent Cells Overview

Invitrogen

Competent Cells, Table of competent cells

Stratagene

Competent Cells

Clontech

Electrocompetent & Chemically Competent E. coli and Yeast Cells

Takara

E. coli Competent Cells