Bacterial strains - DNA

HUJI

Faculty of Science

**Bacterial Strains for DNA Propagation & Cloning**
Bacterial Strain	Company	Uses	Growth requirements/ Antibiotics^r	Documentation
DH5a	Invitrogen Clontech		N one	Invitrogen's manual
Able C	Stratagene	Reduces copy number of plasmid for toxic proteins	Kan15 mgr/ml	A short description
TG-1	Clontech
Sure2	Stratagene	For increased stability of DNA with secondary structures - this strain prevents DNA rearrangments.	Kan15 mgr/ml Tet12.5 mgr/ml Cam34 mgr/ml	A short description
DM-1	Invitrogen	To achieve unmethylated DNA, when problems with partial restriction enzymes activity are observed	Grow in TB buffer (Specified in Description + Protocols )	Description + Protocols A detailed table listing methylation sensitivity of restriction endonucleases in Nucleic Acids Res. 1991 Apr 25;19 Suppl:2045-71 (PDF)
DB3.1	Invirtogen (BRL)	For production of “Gateway”vectors pDest and pDonor vectors containing the toxic gene ccdB	None	A short description
Topp-10	Invirtogen	Cloning of large fragments of DNA or into large vectors (derived from DH10 cells)
NovaBlue	Novagen	K12 strain for cloning with high transformation efficiency, blue-white screening capability, olso contains DE3 for T7 polymerase expression.	Tet12.5 mgr/ml	A short description
Fusion Blue	BD Biosciences (Clontech)	K-12 strain that provides high transformation efficiency paired with blue/white screening capability when used with the appropriate plasmids. The strain carries recA and endA mutations that make it a good host for obtaining high yields of plasmid DNA. Recommended for use with the In-Fusion and Creator cloning systems.	Optional: Tet12.5 mgr/ml Heat shock during transformation should last only 30 sec.
Stbl2	Invirtogen	A derivative of JM109. The mcrA mutation and the mcrBC-hsdRMS-mrr deletion allow cloning of genomic sequences which are methylated. These cells are not capable of blue/white selection with plasmids containing alpha complementation sequences.	Use of a 30°C expression and incubation temperature is important. Heat shock during transformation should last only 25 sec. Stbl2™ Competent Cells which have been transansformed with plasmids should be grown at 30°C overnight in TB	Detailed protocols

**Search for Additional Bacterial Strains**
Novagen	Selection Table of Novagen's Competent Cells Competent cells & Protocols (PDF) Competent Cells Overview
Invitrogen	Competent Cells, Table of competent cells
Stratagene	Competent Cells
Clontech	Electrocompetent & Chemically Competent E. coli and Yeast Cells
Takara	E. coli Competent Cells

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Entries since October 2007

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