Cleavage Sites Table for Fusion Proteins
| PROTEASE |
COMPANY |
Protease
Capture |
| Thrombin Leu-Val-Pro-Arg▼Gly-Ser |
Amersham-Biosciences, Novagen, SIGMA, Roche |
Benzamidine-Agarose |
| Factor
Xa Ile-Glu/Asp-Gly-Arg▼ |
Amersham-Biosciences, New England
Biolabs, Roche |
Benzamidine-Agarose |
| Enterokinase Asp-Asp-Asp-Asp-Lys▼ |
New England Biolabs, Novagen, Roche |
Trypsin Inhibitor-Agarose |
| TEV
protease Glu-Asn-Leu-Tyr-Phe-Gln▼Gly |
Invitrogen – Life Technologies |
Ni-NTA (6His recomb. TEV) |
| PreScission Leu-Glu-Val-Leu-Phe-Gln▼Gly-Pro |
Amersham-Biosciences |
GSTrap for GST fusion enzyme |
| TAGZyme
His-tag removal by Exoproteolytic Digestion |
Quiagen |
Ni-NTA (6His recomb. enzyme) |
| Intein
Site dithiothreitol cleavage |
New England Biolabs |
DTT elimination by dialysis |
| HRV 3C
Protease Leu-Glu-Val-Leu-Phe-Gln▼Gly-Pro |
Novagen |
Ni-NTA (6His recomb. enzyme) |
| SUMO
Protease recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO |
Invitrogen – Life Technologies, LifeSensors |
Ni-NTA (6His recomb. enzyme) |
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