0.6ml 1M tris pH 6.8
4ml 10% SDS
0.02ml EDTA 0.5M pH 8.0
1ml DTT 1M
1ml BphB 1% (solution of 1% Bromophenol Blue)
2.4ml glycerol 100%
0.98ml DDW

Total: 10ml

Stock solutions for preparation of the Sample buffer:
1M Tris pH 6.8
10%SDS
100mM EDTA pH 7.0
1% Bromophenol blue in saturated urea
100% glycerol
DDW