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Protein Expression 
in Mammalian Cells



Selected Papers

  1. Almo SC, Love JD, Better and faster: improvements and optimization for mammalian recombinant protein production, Curr Opin Struct Biol. 2014 26:39-43
  2. Bandaranayake AD and Almo SC, Recent advances in mammalian protein production, FEBS Lett. 2014 588(2):253-260
  3. Khan KH, Gene expression in Mammalian cells and its applications. Adv. Pharm. Bull. 2013 3(2):257-263
  4. Aricescu AR and Owens RJ., Expression of recombinant glycoproteins in mammalian cells: towards anintegrative approach to structural biology. Curr Opin Struct Biol 2013 23(3):345-56
Expression System
Bacground
Cell Lines
Vectors in Use
Related Procedures
General Procedures
   Mammalian cell lines - a list by Cell-culture Databse  

1. Standard transfection protocol
2. Cryo preservation of mammalian cells
3. Polycistronic expression of proteins in Eukaryotic systems using self processing peptides (1 , 2 , 3 , 4 ,)
Transient Expression
        
Stable Expression
        
Flip In
 Using the Flip-In system for isolating protein complexes: 1 , 2 ,     Flip In system manual

Lentivirus

      

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entries since October 2013

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