LOCUS pET-21d(+) 5440 bp DNA circular 3-MAY-2002
SOURCE
ORGANISM
COMMENT http://www.informaxinc.com
COMMENT This file is created by Vector NTI
http://www.informaxinc.com/
COMMENT ORIGDB|GenBank
COMMENT VNTDBDATE|265738957|
COMMENT VNTNAME|pET-21d(+)|
COMMENT VNTAUTHORNAME|Demo User|
COMMENT VNTOAUTHORNAME|Larissa Wiskowski|
COMMENT VNTOAUTHORTEL|608-238-6110|
COMMENT VNTOAUTHORFAX|608-238-1388|
COMMENT VNTOAUTHOREML|Novatech@novagen.com|
COMMENT VNTOAUTHORWWW|www.novagen.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT (SXF
COMMENT (CGexDoc "pET-21d(+)" 0 5440
COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList)
COMMENT (CObList))
COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 5 10000 50 0 1 0
COMMENT (CHomObj 0 0 0 3 75)
COMMENT (CWordArray 47 49 50 48 51 52 81 0 1 3 4 82 5 83 2 6 8 7 9 61 10 11 12
COMMENT 13 14 15 16 17 18 19 84 20 85 21 22 86 87 88 23 54 62 24 89 25 26 55 91
COMMENT 27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 57 38 36 39 40 41 42 43
COMMENT 95 44 59 99 96 97 45 100 46) (CWordArray)
COMMENT (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI"
COMMENT "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg")
COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0
COMMENT 4294967295) (CMolPar 0 0 0 0 0 1 5440 0 0 0 0 0 0 0 0) (CStringList)
COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7)
COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7)
COMMENT (CObList
COMMENT #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 4 1042 0 2977 0 3477 0
COMMENT 4723 0) #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 159 0)
COMMENT #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 199 0)
COMMENT #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0)
COMMENT #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 193 0)
COMMENT #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 174 0)
COMMENT #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 235 0)
COMMENT #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 4301 0)
COMMENT #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0)
COMMENT #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0))
COMMENT (CObList
COMMENT #10=(CFSignal (CObList) "T7 promoter" 30 0 1 308 324 0 (CStringList)
COMMENT (CStringList) 1 1 1 1 "")
COMMENT #11=(CFSignal (CObList) "T7 tag" 31 0 1 205 237 0 (CStringList)
COMMENT (CStringList) 1 1 1 1 "")
COMMENT #12=(CFSignal (CObList) "lac operator" 4 0 1 281 305 0 (CStringList)
COMMENT (CStringList) 1 1 1 1 "")
COMMENT #13=(CFSignal (CObList) "T7 terminator" 43 0 1 26 72 0 (CStringList)
COMMENT (CStringList) 1 1 1 1 "")
COMMENT #14=(CFSignal (CObList) "His tag" 31 0 1 140 157 0 (CStringList)
COMMENT (CStringList) 1 1 1 1 "")
COMMENT #15=(CFSignal (CObList) "lac I" 4 0 0 711 1790 0 (CStringList)
COMMENT (CStringList) 1 1 1 1 "")
COMMENT #16=(CFSignal (CObList) "ColE1 pBR322 origin" 33 0 1 3224 3224 0
COMMENT (CStringList) (CStringList) 1 1 1 1 "")
COMMENT #17=(CFSignal (CObList) "bla (Ap) sequence" 4 0 1 3985 4842 0
COMMENT (CStringList) (CStringList) 1 1 1 1 "")
COMMENT #18=(CFSignal (CObList) "f1 origin" 33 0 1 4974 5429 0 (CStringList)
COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList)
COMMENT (CObList) (CObList)
COMMENT (CTextView 0
COMMENT #19=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180)
COMMENT (CObjectList
COMMENT #20=(CRefLinePar
COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pET-21d(+)" 2) 5
COMMENT "" 0 4)
COMMENT #21=(CFolderPar
COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178)
COMMENT (CObjectList
COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "DNA 'pET-21d(+)'" 1)
COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "Currently local object. Original author: Larissa Wiskowski"
COMMENT 1)
COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "Creation Date: ? (no data)" 1)
COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "Last Modified: 05/03/02 04:22PM" 1)
COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "length: 5440 bp" 1)
COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "storage type: Basic" 1)
COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "form: Circular" 1))) "General Description")
COMMENT #29=(CFolderPar
COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178)
COMMENT (CObjectList
COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "Original Source Database: GenBank" 1)))
COMMENT "Standard Fields")
COMMENT #31=(CFolderPar
COMMENT (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178)
COMMENT (CObjectList
COMMENT #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "Larissa Wiskowski" 1)
COMMENT #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "Novagen Inc." 1)
COMMENT #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "TEL: 608-238-6110 FAX: 608-238-1388" 1)
COMMENT #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "EMAIL: http://www.merckbiosciences.co.uk/"mailto:Novatech@novagen.com/""
COMMENT 1)
COMMENT #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)
COMMENT "WWW: http://www.merckbiosciences.co.uk/"http://www.novagen.com/""
COMMENT 1))) "Original Author")
COMMENT #37=(CRefLinePar
COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 ""
COMMENT 0 0)
COMMENT #38=(CFolderPar
COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178)
COMMENT (CObjectList
COMMENT #39=(CFolderPar
COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178)
COMMENT (CObjectList
COMMENT #40=(CFolderPar
COMMENT (CGroupPar
COMMENT (CParagraph 281 (3 #12# 0) 1 2 2 0 327)
COMMENT (CObjectList
COMMENT #41=(CLinePar
COMMENT (CParagraph 0 (0 0) 1 2 3 0 180)
COMMENT "Start: 281 End: 305 (Complementary)"
COMMENT 1))) "lac operator")
COMMENT #42=(CFolderPar
COMMENT (CGroupPar
COMMENT (CParagraph 711 (3 #15# 0) 1 2 2 0 327)
COMMENT (CObjectList
COMMENT #43=(CLinePar
COMMENT (CParagraph 0 (0 0) 1 2 3 0 180)
COMMENT "Start: 711 End: 1790" 1))) "lac I")
COMMENT #44=(CFolderPar
COMMENT (CGroupPar
COMMENT (CParagraph 3985 (3 #17# 0) 1 2 2 0 327)
COMMENT (CObjectList
COMMENT #45=(CLinePar
COMMENT (CParagraph 0 (0 0) 1 2 3 0 180)
COMMENT "Start: 3985 End: 4842 (Complementary)"
COMMENT 1))) "bla (Ap) sequence")))
COMMENT "CDS (3 total)")
COMMENT #46=(CFolderPar
COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178)
COMMENT (CObjectList
COMMENT #47=(CFolderPar
COMMENT (CGroupPar
COMMENT (CParagraph 308 (3 #10# 0) 1 2 2 0 194)
COMMENT (CObjectList
COMMENT #48=(CLinePar
COMMENT (CParagraph 0 (0 0) 1 2 3 0 180)
COMMENT "Start: 308 End: 324 (Complementary)"
COMMENT 1))) "T7 promoter")))
COMMENT "Promoter Prokaryotic (1 total)")
COMMENT #49=(CFolderPar
COMMENT (CGroupPar (CParagraph 31 (7 31 0) 1 1 1 0 178)
COMMENT (CObjectList
COMMENT #50=(CFolderPar
COMMENT (CGroupPar
COMMENT (CParagraph 140 (3 #14# 0) 1 2 2 0 194)
COMMENT (CObjectList
COMMENT #51=(CLinePar
COMMENT (CParagraph 0 (0 0) 1 2 3 0 180)
COMMENT "Start: 140 End: 157 (Complementary)"
COMMENT 1))) "His tag")
COMMENT #52=(CFolderPar
COMMENT (CGroupPar
COMMENT (CParagraph 205 (3 #11# 0) 1 2 2 0 194)
COMMENT (CObjectList
COMMENT #53=(CLinePar
COMMENT (CParagraph 0 (0 0) 1 2 3 0 180)
COMMENT "Start: 205 End: 237 (Complementary)"
COMMENT 1))) "T7 tag")))
COMMENT "Protein Binding Site (2 total)")
COMMENT #54=(CFolderPar
COMMENT (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178)
COMMENT (CObjectList
COMMENT #55=(CFolderPar
COMMENT (CGroupPar
COMMENT (CParagraph 3224 (3 #16# 0) 1 2 2 0 194)
COMMENT (CObjectList
COMMENT #56=(CLinePar
COMMENT (CParagraph 0 (0 0) 1 2 3 0 180)
COMMENT "Start: 3224 End: 3224 (Complementary)"
COMMENT 1))) "ColE1 pBR322 origin")
COMMENT #57=(CFolderPar
COMMENT (CGroupPar
COMMENT (CParagraph 4974 (3 #18# 0) 1 2 2 0 194)
COMMENT (CObjectList
COMMENT #58=(CLinePar
COMMENT (CParagraph 0 (0 0) 1 2 3 0 180)
COMMENT "Start: 4974 End: 5429 (Complementary)"
COMMENT 1))) "f1 origin")))
COMMENT "Replication Origin (2 total)")
COMMENT #59=(CFolderPar
COMMENT (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178)
COMMENT (CObjectList
COMMENT #60=(CFolderPar
COMMENT (CGroupPar
COMMENT (CParagraph 26 (3 #13# 0) 1 2 2 0 194)
COMMENT (CObjectList
COMMENT #61=(CLinePar
COMMENT (CParagraph 0 (0 0) 1 2 3 0 180)
COMMENT "Start: 26 End: 72 (Complementary)"
COMMENT 1))) "T7 terminator")))
COMMENT "Terminator (1 total)"))) "Feature Map")
COMMENT #62=(CFolderPar
COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178)
COMMENT (CObjectList
COMMENT #63=(CRSFolderPar
COMMENT (CFolderPar
COMMENT (CGroupPar (CParagraph 24569416 (8 0) 1 1 1 0 178)
COMMENT (CObjectList
COMMENT #64=(CGroupPar
COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180)
COMMENT (CObjectList
COMMENT #65=(CLinePar
COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191)
COMMENT " N1: 1042 " 1)
COMMENT #66=(CLinePar
COMMENT (CParagraph 0 (1 #0# 2) 1 2 2 0 191)
COMMENT " N2: 2977 " 1)
COMMENT #67=(CLinePar
COMMENT (CParagraph 0 (1 #0# 3) 1 2 2 0 191)
COMMENT " N3: 3477 " 1)
COMMENT #68=(CLinePar
COMMENT (CParagraph 0 (1 #0# 4) 1 2 2 0 191)
COMMENT " N4: 4723 " 1))))) "ApaLI: 4 sites") 1
COMMENT 5 "GTGCAC" "CACGTG")
COMMENT #69=(CRSFolderPar
COMMENT (CFolderPar
COMMENT (CGroupPar (CParagraph 35027776 (8 0) 1 1 1 0 178)
COMMENT (CObjectList
COMMENT #70=(CGroupPar
COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180)
COMMENT (CObjectList
COMMENT #71=(CLinePar
COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191)
COMMENT " N1: 159 " 1))))) "AvaI: 1 site") 1 5
COMMENT "CYCGRG" "GRGCYC")
COMMENT #72=(CRSFolderPar
COMMENT (CFolderPar
COMMENT (CGroupPar (CParagraph 35001896 (8 0) 1 1 1 0 178)
COMMENT (CObjectList
COMMENT #73=(CGroupPar
COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180)
COMMENT (CObjectList
COMMENT #74=(CLinePar
COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191)
COMMENT " N1: 199 " 1))))) "BamHI: 1 site") 1 5
COMMENT "GGATCC" "CCTAGG")
COMMENT #75=(CRSFolderPar
COMMENT (CFolderPar
COMMENT (CGroupPar (CParagraph 35014312 (8 0) 1 1 1 0 178)
COMMENT (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT"
COMMENT "TAGCTA")
COMMENT #76=(CRSFolderPar
COMMENT (CFolderPar
COMMENT (CGroupPar (CParagraph 35001096 (8 0) 1 1 1 0 178)
COMMENT (CObjectList
COMMENT #77=(CGroupPar
COMMENT (CParagraph 0 (10 #4# 0) 1 2 2 0 180)
COMMENT (CObjectList
COMMENT #78=(CLinePar
COMMENT (CParagraph 0 (1 #4# 1) 1 2 2 0 191)
COMMENT " N1: 193 " 1))))) "EcoRI: 1 site") 1 5
COMMENT "GAATTC" "CTTAAG")
COMMENT #79=(CRSFolderPar
COMMENT (CFolderPar
COMMENT (CGroupPar (CParagraph 35007344 (8 0) 1 1 1 0 178)
COMMENT (CObjectList
COMMENT #80=(CGroupPar
COMMENT (CParagraph 0 (10 #5# 0) 1 2 2 0 180)
COMMENT (CObjectList
COMMENT #81=(CLinePar
COMMENT (CParagraph 0 (1 #5# 1) 1 2 2 0 191)
COMMENT " N1: 174 " 1))))) "HindIII: 1 site") 1
COMMENT 5 "AAGCTT" "TTCGAA")
COMMENT #82=(CRSFolderPar
COMMENT (CFolderPar
COMMENT (CGroupPar (CParagraph 24515120 (8 0) 1 1 1 0 178)
COMMENT (CObjectList
COMMENT #83=(CGroupPar
COMMENT (CParagraph 0 (10 #6# 0) 1 2 2 0 180)
COMMENT (CObjectList
COMMENT #84=(CLinePar
COMMENT (CParagraph 0 (1 #6# 1) 1 2 2 0 191)
COMMENT " N1: 235 " 1))))) "NcoI: 1 site") 1 5
COMMENT "CCATGG" "GGTACC")
COMMENT #85=(CRSFolderPar
COMMENT (CFolderPar
COMMENT (CGroupPar (CParagraph 35025536 (8 0) 1 1 1 0 178)
COMMENT (CObjectList
COMMENT #86=(CGroupPar
COMMENT (CParagraph 0 (10 #7# 0) 1 2 2 0 180)
COMMENT (CObjectList
COMMENT #87=(CLinePar
COMMENT (CParagraph 0 (1 #7# 1) 1 2 2 0 191)
COMMENT " N1: 4301 " 1))))) "PstI: 1 site") 5 1
COMMENT "CTGCAG" "GACGTC")
COMMENT #88=(CRSFolderPar
COMMENT (CFolderPar
COMMENT (CGroupPar (CParagraph 24592208 (8 0) 1 1 1 0 178)
COMMENT (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG"
COMMENT "GGGCCC")
COMMENT #89=(CRSFolderPar
COMMENT (CFolderPar
COMMENT (CGroupPar (CParagraph 24593808 (8 0) 1 1 1 0 178)
COMMENT (CObjectList)) "XmaI: 0 sites") 1 5 "CCCGGG"
COMMENT "GGGCCC"))) "Restriction Map: ")
COMMENT #90=(CFolderPar
COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList))
COMMENT "Annotations"))))
COMMENT (CGraphView
COMMENT (CWStyleSheet
COMMENT (CObjectList
COMMENT #91=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1
COMMENT (LOGBRUSH 0 16777215 0) 1
COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 1
COMMENT 2 "?" 0)
COMMENT #92=(CWidgetStyle "Mark 1" 0 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 0)
COMMENT #93=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 1
COMMENT 65535 "@N (@H)" 0)
COMMENT #94=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8437824) 1 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.611111
COMMENT 0 1 5 "@N (@S)" 0)
COMMENT #95=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 4227264) 1 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1
COMMENT "@N" 0)
COMMENT #96=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48
COMMENT "@F bp (molecule @L bp)" 0)
COMMENT #97=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1
COMMENT "Fragment of @N" 0)
COMMENT #98=(CWidgetStyle "Molecule Label 2" 0 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 0.888889 0 1
COMMENT 16 "@L bp" 0)
COMMENT #99=(CWidgetStyle "Molecule Label 1" 0 0 1
COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.833333 0 1 1
COMMENT "@N" 0)
COMMENT #100=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1
COMMENT (LOGBRUSH 2 8388608 5) 0 0 0)
COMMENT #101=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 26367 0)
COMMENT 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0))
COMMENT #102=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0
COMMENT 0 0)
COMMENT #103=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1
COMMENT (LOGBRUSH 0 16728256 0) 0 0 1 (LOGSHAPE 8 1 0 0 0))
COMMENT #104=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0
COMMENT 0)
COMMENT #105=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0)
COMMENT 0 0 0)
COMMENT #106=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1
COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808))
COMMENT #107=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0)
COMMENT #108=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0)
COMMENT #109=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0)
COMMENT #110=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0)
COMMENT #111=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0)
COMMENT #112=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0)
COMMENT #113=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0)
COMMENT #114=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0)
COMMENT #115=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0)
COMMENT #116=(CWidgetStyle "Void" 0 0 0 0 0)
COMMENT #117=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman")
COMMENT 0.611111 128 1 5 "@N (@S)" 0)
COMMENT #118=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0
COMMENT 1 3 "@T @N " 0)
COMMENT #119=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0)
COMMENT #120=(CWidgetStyle "Annotation" 0 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0
COMMENT 0 0)
COMMENT #121=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1
COMMENT "@N" 0)
COMMENT #122=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1
COMMENT (LOGBRUSH 0 16777215 0) 0 0 0)
COMMENT #123=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1
COMMENT "@N" 0)
COMMENT #124=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1
COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman")
COMMENT 0.611111 0 1 65535 "@N" 0)) 0.164315 1.74233 0.164644 2.53336
COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67
COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 42.2507 42.2507 39 39 0.1 0) 1
COMMENT 1 0 1 1
COMMENT (mapper: 19.4759 -21.305 42.2507 42.2507 0.01 10 -1 5440 5440 1 0 0)
COMMENT #125=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 720906 100)
COMMENT (CObjectList
COMMENT #126=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1140 100)
COMMENT (CObjectList
COMMENT #127=(CAxis
COMMENT (CWideLine
COMMENT (CWidget 0 (0 0) 1 2 0 0 #105# 24735604 0)
COMMENT (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27534
COMMENT 6.27334 1 0.021361) 0.115316)
COMMENT #128=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #99# 393238 100)
COMMENT (LOGPEN 0 0 4227264) 1
COMMENT (LOGFONT 61 23 0 0 700 0 0 0 0 3 2 1 34 "Arial")
COMMENT 1.74233 0.833333 0 "pET-21d(+)" "@N" 1 0 0.871165 0
COMMENT -10 5.91706 1.42009 Nil)
COMMENT #129=(CLabel
COMMENT (CWidget 1002 (0 0) 1 2 0 0 #98# 35357208 100)
COMMENT (LOGPEN 0 0 4227264) 1
COMMENT (LOGFONT 65 24 0 0 400 0 0 0 0 3 2 1 49
COMMENT "Courier New") 1.74233 0.888889 0 "5440 bp" "@L bp"
COMMENT 16 0 -0.871165 0 -10.8 3.45556 1.51477 Nil)))
COMMENT #130=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1361 100)
COMMENT (CObjectList
COMMENT #131=(CGroupWidget
COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil -330 100)
COMMENT (CObjectList
COMMENT #132=(CScratch
COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #114# 0 100)
COMMENT (LOGPEN 0 8 8388608) 10 1 5.9503 1.9
COMMENT 0.0821575 1)
COMMENT #133=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #95# 131077 100)
COMMENT (LOGPEN 0 0 4227264) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 34
COMMENT "Arial") 1.74233 0.611111 0 "lac operator"
COMMENT "@N" 1 13.8682 3.48466 0 -10 3.88159 0.94673
COMMENT #132#)
COMMENT #134=(CWideArrow
COMMENT (CWideLine
COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #101# 0 100)
COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 26367 0) 1
COMMENT 4.20903 5.45444 1 0.0821575) 0.8 1.8 0)
COMMENT #135=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #95# 12911008 100)
COMMENT (LOGPEN 0 0 4227264) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 34
COMMENT "Arial") 1.74233 0.611111 0 "lac I" "@N" 1
COMMENT 12.7907 0 0 -10 1.42009 0.94673 #134#)
COMMENT #136=(CWideArrow
COMMENT (CWideLine
COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #101# 0 100)
COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 26367 0) 1
COMMENT 0.689589 1.679 1 0.0821575) 0.8 1.8 1)
COMMENT #137=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #95# 35382400 100)
COMMENT (LOGPEN 0 0 4227264) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 34
COMMENT "Arial") 1.74233 0.611111 0
COMMENT "bla (Ap) sequence" "@N" 1 -14.8339 5.22699 0
COMMENT -10 5.9644 0.94673 #136#)))
COMMENT #138=(CGroupWidget
COMMENT (CWidget 30 (7 30 0) 1 2 0 0 Nil -772 100)
COMMENT (CObjectList
COMMENT #139=(CPromoterArrow
COMMENT (CLine
COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #111# 0 100)
COMMENT (LOGPEN 0 4 128) 6 0.835685 5.91917 6.00643)
COMMENT 0.4 0.0821575 0.915685 1.8 1.8 1)
COMMENT #140=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #95# 24837188 100)
COMMENT (LOGPEN 0 0 4227264) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 34
COMMENT "Arial") 1.74233 0.611111 0 "T7 promoter"
COMMENT "@N" 1 13.3657 5.22699 0 -10 3.97627 0.94673
COMMENT #139#)))
COMMENT #141=(CGroupWidget
COMMENT (CWidget 31 (7 31 0) 1 2 0 0 Nil -698 100)
COMMENT (CObjectList
COMMENT #142=(CScratch
COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #109# 0 100)
COMMENT (LOGPEN 0 6 16711680) 8 1 6.03794 1.9
COMMENT 0.0821575 1)
COMMENT #143=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #95# 25489872 100)
COMMENT (LOGPEN 0 0 4227264) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 34
COMMENT "Arial") 1.74233 0.611111 0 "T7 tag" "@N" 1
COMMENT 9.94201 8.71165 0 -10 2.17748 0.94673 #142#)
COMMENT #144=(CScratch
COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #109# 0 100)
COMMENT (LOGPEN 0 6 16711680) 8 1 6.1129 1.9
COMMENT 0.0821575 1)
COMMENT #145=(CLabel (CWidget 0 (0 0) 1 2 0 0 #95# 1 100)
COMMENT (LOGPEN 0 0 4227264) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 34
COMMENT "Arial") 1.74233 0.611111 0 "His tag" "@N" 1
COMMENT 4.4958 17.4233 0 -10 2.4615 0.94673 #144#)))
COMMENT #146=(CGroupWidget
COMMENT (CWidget 33 (7 33 0) 1 2 0 0 Nil -478 100)
COMMENT (CObjectList
COMMENT #147=(CScratch
COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #114# 0 100)
COMMENT (LOGPEN 0 8 8388608) 10 1 2.55655 1.9
COMMENT 0.0821575 1)
COMMENT #148=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #95# 12911144 100)
COMMENT (LOGPEN 0 0 4227264) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 34
COMMENT "Arial") 1.74233 0.611111 0
COMMENT "ColE1 pBR322 origin" "@N" 1 -11.3459 -10.454
COMMENT 0 -10 6.91113 0.94673 #147#)
COMMENT #149=(CWideArrow
COMMENT (CWideLine
COMMENT (CWidget 0 (3 #18# 0) 1 2 0 0 #101# 0 100)
COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 26367 0) 1
COMMENT 0.0126847 0.538525 1 0.0821575) 0.8 1.8 1)
COMMENT #150=(CLabel (CWidget 0 (0 0) 1 2 0 0 #95# 423 100)
COMMENT (LOGPEN 0 0 4227264) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 34
COMMENT "Arial") 1.74233 0.611111 0 "f1 origin" "@N"
COMMENT 1 -5.61834 12.1963 0 -10 2.74552 0.94673
COMMENT #149#)))
COMMENT #151=(CGroupWidget
COMMENT (CWidget 43 (7 43 0) 1 2 0 0 Nil -404 100)
COMMENT (CObjectList
COMMENT #152=(CLine
COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #114# 720906
COMMENT 100) (LOGPEN 0 8 8388608) 10 0.835685
COMMENT 6.19016 6.24436)
COMMENT #153=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #95# 393235 100)
COMMENT (LOGPEN 0 0 4227264) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 34
COMMENT "Arial") 1.74233 0.611111 0 "T7 terminator"
COMMENT "@N" 1 4.49228 19.1656 0 -10 4.35496 0.94673
COMMENT #152#)))))
COMMENT #154=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -257 100)
COMMENT (CObjectList
COMMENT #155=(CGroupWidget
COMMENT (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -330 100)
COMMENT (CObjectList
COMMENT #156=(CScratch
COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #112# 0 100)
COMMENT (LOGPEN 0 6 0) 8 1 6.09099 1.9 0.0821575 1)
COMMENT #157=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #117# 35389188 100)
COMMENT (LOGPEN 0 0 8421376) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 18
COMMENT "Times New Roman") 1.74233 0.611111 128
COMMENT "{\\i Ava}I (159)" "@N (@S)" 5 5.18757 15.681
COMMENT 0 -10 3.78692 0.94673 #156#)))
COMMENT #158=(CGroupWidget
COMMENT (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -1287 100)
COMMENT (CObjectList
COMMENT #159=(CScratch
COMMENT (CWidget 1 (1 #2# 1) 1 2 0 0 #112# 0 100)
COMMENT (LOGPEN 0 6 0) 8 1 6.04486 1.9 0.0821575 1)
COMMENT #160=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #117# 35359608 100)
COMMENT (LOGPEN 0 0 8421376) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 18
COMMENT "Times New Roman") 1.74233 0.611111 128
COMMENT "{\\i Bam}HI (199)" "@N (@S)" 5 8.80912
COMMENT 10.454 0 -10 4.63898 0.94673 #159#)))
COMMENT #161=(CGroupWidget
COMMENT (CWidget 1 (10 #4# 0) 1 2 0 0 Nil -183 100)
COMMENT (CObjectList
COMMENT #162=(CScratch
COMMENT (CWidget 1 (1 #4# 1) 1 2 0 0 #112# 0 100)
COMMENT (LOGPEN 0 6 0) 8 1 6.05178 1.9 0.0821575 1)
COMMENT #163=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #117# 24592208 100)
COMMENT (LOGPEN 0 0 8421376) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 18
COMMENT "Times New Roman") 1.74233 0.611111 128
COMMENT "{\\i Eco}RI (193)" "@N (@S)" 5 5.68113
COMMENT 12.1963 0 -10 4.26028 0.94673 #162#)))
COMMENT #164=(CGroupWidget
COMMENT (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -551 100)
COMMENT (CObjectList
COMMENT #165=(CScratch
COMMENT (CWidget 1 (1 #5# 1) 1 2 0 0 #112# 0 100)
COMMENT (LOGPEN 0 6 0) 8 1 6.07369 1.9 0.0821575 1)
COMMENT #166=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100)
COMMENT (LOGPEN 0 0 8421376) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 18
COMMENT "Times New Roman") 1.74233 0.611111 128
COMMENT "{\\i Hin}dIII (174)" "@N (@S)" 5 5.74995
COMMENT 13.9386 0 -10 4.73365 0.94673 #165#)))
COMMENT #167=(CGroupWidget
COMMENT (CWidget 1 (10 #6# 0) 1 2 0 0 Nil -625 100)
COMMENT (CObjectList
COMMENT #168=(CScratch
COMMENT (CWidget 1 (1 #6# 1) 1 2 0 0 #112# 0 100)
COMMENT (LOGPEN 0 6 0) 8 1 6.00335 1.9 0.0821575 1)
COMMENT #169=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #117# 25418064 100)
COMMENT (LOGPEN 0 0 8421376) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 18
COMMENT "Times New Roman") 1.74233 0.611111 128
COMMENT "{\\i Nco}I (235)" "@N (@S)" 5 11.9956
COMMENT 6.96932 0 -10 3.78692 0.94673 #168#)))
COMMENT #170=(CGroupWidget
COMMENT (CWidget 1 (10 #7# 0) 1 2 0 0 Nil -625 100)
COMMENT (CObjectList
COMMENT #171=(CScratch
COMMENT (CWidget 1 (1 #7# 1) 1 2 0 0 #112# 0 100)
COMMENT (LOGPEN 0 6 0) 8 1 1.3146 1.9 0.0821575 1)
COMMENT #172=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #117# 12911048 100)
COMMENT (LOGPEN 0 0 8421376) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 18
COMMENT "Times New Roman") 1.74233 0.611111 128
COMMENT "{\\i Pst}I (4301)" "@N (@S)" 5 -13.6648
COMMENT 3.48466 0 -10 3.97627 0.94673 #171#)))
COMMENT #173=(CGroupWidget
COMMENT (CWidget 4 (10 #0# 0) 1 2 0 0 Nil -110 100)
COMMENT (CObjectList
COMMENT #174=(CScratch
COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #112# 0 100)
COMMENT (LOGPEN 0 6 0) 8 1 5.07275 1.9 0.0821575 1)
COMMENT #175=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #94# 1598667368 100)
COMMENT (LOGPEN 0 0 8437824) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 18
COMMENT "Times New Roman") 1.74233 0.611111 0
COMMENT "{\\i Apa}LI (1042)" "@N (@S)" 5 14.239
COMMENT 1.74233 0 -10 4.73365 0.94673 #174#)
COMMENT #176=(CScratch
COMMENT (CWidget 2 (1 #0# 2) 1 2 0 0 #112# 0 100)
COMMENT (LOGPEN 0 6 0) 8 1 2.84138 1.9 0.0821575 1)
COMMENT #177=(CLabel (CWidget 0 (0 0) 1 2 0 0 #94# 0 100)
COMMENT (LOGPEN 0 0 8437824) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 18
COMMENT "Times New Roman") 1.74233 0.611111 0
COMMENT "{\\i Apa}LI (2977)" "@N (@S)" 5 -6.68481
COMMENT -12.1963 0 -10 4.73365 0.94673 #176#)
COMMENT #178=(CScratch
COMMENT (CWidget 3 (1 #0# 3) 1 2 0 0 #112# 0 100)
COMMENT (LOGPEN 0 6 0) 8 1 2.2648 1.9 0.0821575 1)
COMMENT #179=(CLabel (CWidget 0 (0 0) 1 2 0 0 #94# 0 100)
COMMENT (LOGPEN 0 0 8437824) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 18
COMMENT "Times New Roman") 1.74233 0.611111 0
COMMENT "{\\i Apa}LI (3477)" "@N (@S)" 5 -12.4486
COMMENT -6.96932 0 -10 4.73365 0.94673 #178#)
COMMENT #180=(CScratch
COMMENT (CWidget 4 (1 #0# 4) 1 2 0 0 #112# 0 100)
COMMENT (LOGPEN 0 6 0) 8 1 0.827968 1.9 0.0821575 1)
COMMENT #181=(CLabel
COMMENT (CWidget 0 (0 0) 1 2 0 0 #94# 24506848 100)
COMMENT (LOGPEN 0 0 8437824) 1
COMMENT (LOGFONT 44 16 0 0 400 0 0 0 0 3 2 1 18
COMMENT "Times New Roman") 1.74233 0.611111 0
COMMENT "{\\i Apa}LI (4723)" "@N (@S)" 5 -11.6808
COMMENT 8.71165 0 -10 4.73365 0.94673 #180#)))))
COMMENT #182=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -846 100)
COMMENT (CObjectList))
COMMENT #183=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -698 100)
COMMENT (CObjectList))))) (CSeqView 10 5 (CObjectList) (CObList) 1)
COMMENT (CObList) 0 (CStringList) 0 0 (CObList)))
FEATURES Location/Qualifiers
promoter complement(308..324)
/vntifkey="30"
/label=T7\promoter
protein_bind complement(205..237)
/vntifkey="31"
/label=T7\tag
CDS complement(281..305)
/vntifkey="4"
/label=lac\operator
terminator complement(26..72)
/vntifkey="43"
/label=T7\terminator
protein_bind complement(140..157)
/vntifkey="31"
/label=His\tag
CDS 711..1790
/vntifkey="4"
/label=lac\I
rep_origin complement(3224..3224)
/vntifkey="33"
/label=ColE1\pBR322\origin
CDS complement(3985..4842)
/vntifkey="4"
/label=bla\(Ap)\sequence
rep_origin complement(4974..5429)
/vntifkey="33"
/label=f1\origin
BASE COUNT 1287 a 1445 c 1441 g 1267 t
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatggt
241 atatctcctt cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca
301 attcccctat agtgagtcgt attaatttcg cgggatcgag atctcgatcc tctacgccgg
361 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
421 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
481 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
541 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
601 ggagtcgcat aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc
661 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
721 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
781 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
841 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
901 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
961 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
1021 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
1081 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
1141 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
1201 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
1261 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
1321 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
1381 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
1441 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
1501 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
1561 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
1621 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
1681 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
1741 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
1801 gcaattaatg taagttagct cactcattag gcaccgggat ctcgaccgat gcccttgaga
1861 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
1921 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
1981 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
2041 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
2101 gagaagcagg ccattatcgc cggcatggcg gccccacggg tgcgcatgat cgtgctcctg
2161 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg
2221 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca
2281 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc
2341 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
2401 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
2461 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
2521 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
2581 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc
2641 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
2701 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc
2761 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
2821 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
2881 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
2941 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc ggtgtgaaat
3001 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
3061 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
3121 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
3181 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
3241 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
3301 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
3361 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
3421 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3481 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
3541 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
3601 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
3661 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
3721 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
3781 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
3841 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
3901 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
3961 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
4021 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
4081 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
4141 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
4201 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
4261 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
4321 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
4381 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
4441 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
4501 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
4561 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
4621 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
4681 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
4741 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
4801 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
4861 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
4921 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgaaattgt
4981 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
5041 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
5101 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
5161 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
5221 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
5281 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
5341 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
5401 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
//