ýVolatile buffer systems used in ion exchange ýýchromatography

The Protein Purification Facility
The Alexander Silberman Institute of Life Sciences
The Hebrew University of Jerusalem
Dr. Mario Lebendiker
mariol@mail.ls.huji.ac.il Tel: 972-2-6586920 FAX: 972-2-6758963

Recommended Volatile Buffers Systems for Ion Exchange Chromatography

Recommended volatile buffer substances for ion exchange chromatography. According to Amersham Biosciences catalogue

pH range	Buffer system	Counter-ion	pK-values for buffering ions
2.0	Formic acid	H+	3.75
2.3-3.5	Pyridine/formic acid	HCOO^-	3.75; 5.25
3.0-5.0	Trimethylamine/formic acid	HCOO^-	3.75; 9.25
3.0-6.0	Pyridine/acetic acid	CH₃COO^-	4.76; 5.25
4.0-6.0	Trimethylamine/acetic acid	CH₃COO^-	4.76; 9.25
6.8-8.8	Trimethylamine/HCI	CI-	9.25
7.0-8.5	Ammonia/formic acid	HCOO^-	3.75; 9.25
8.5-10.0	Ammonia/acetic acid	CH₃COO^-	4.76; 9.25
7.0-12.0	Trimethylamine/carbonate	CO₃^2-	6.50; 9.25
7.9	Ammonium bicarbonate	HCO₃^-	6.50; 9.25
8.0-9.5	Ammonium carbonate/ammonia	CO₃^2-	6.50; 9.25
8.5-10.5	Ethanolamine/HCI	CI^-	10.0
8.5	Ammonium carbonate	CO₃^2-	6.50; 9.25

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Dr. Mario Lebendiker The Protein Purification Facility
The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem
mariol@mail.ls.huji.ac.il Tel: 972-2-6586920 FAX: 972-2-6758963