Stop Codons
Background: The presence of a stop codon is an indispensable signal for termination of mRNA translation. The genetic code includes three stop codons: UAA, UAG and UGA.   E. coli displays a strong bias towards the UAA codon, and translational termination efficiency is further improved by the prolonged UAAU sequence. Alternatively, two consecutive stop codons may ensure termination.
Note: if the cDNA of the target gene is cloned upstream to a fusion tag/protein, stop codons between the target protein and the fusion partner must be omitted.
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