Background: The presence of a stop codon
is an indispensable signal for termination of mRNA translation. The genetic
code includes three stop codons: UAA, UAG and UGA. E. coli
displays a strong bias towards the UAA codon, and translational termination
efficiency is further improved by the prolonged UAAU sequence. Alternatively,
two consecutive stop codons may ensure termination.
Note: if the cDNA of the target
gene is cloned upstream to a fusion tag/protein, stop codons between the
target protein and the fusion partner must be omitted.
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