1. Take 1ul of your super-coiled
DNA (0.1ug-0.5ug) directly into 40ul of competent bacteria in eppendorf
(bacteria can be PEG competent or anything BUT electrocompetent)
2. Incubate for 10 min on ice
3. Heat shock for 45'' at 42oC
4. Cool on ice for 2 min
5. Plate all tube content on a LB-agar plate containing the appropriate antibiotics
6. Incubate over-night at 37oC.