Quick Transformation
When you have no time for a real transformation, and you have super-coiled DNA, in high concentration, you may use this quick procedure. please note that the efficiency of this procedure is low, and may vary according to your vector.

1. Take 1ul of your super-coiled DNA (0.1ug-0.5ug) directly into 40ul of competent bacteria in eppendorf tube
(bacteria can be PEG competent or anything BUT electrocompetent)
2. Incubate for 10 min on ice
3. Heat shock for 45'' at 42oC
4. Cool on ice for 2 min
5. Plate all tube content on a LB-agar plate containing the appropriate antibiotics
6. Incubate over-night at 37oC.